New Services

High Affinity Screening

Never miss a high-affinity antibody again. Many methods are only applied after final antibody selection. ImmunoPrecise allows screening at a very early stage without need for pure, known quantities of mAb and without the need of Surface Plasmon Resonance and BEFORE any clones are disregarded during the early selection process.

Promotions

Amazon.com gift card Spring promotion

$100 Amazon.com gift card Spring promotion for every new custom mAb project started from Feb 1st until Jun 30th, 2011.

Accolades

Customer Feedback

Listen to what IPA's existing & past customers have to say about our products & custom antibody development services.

Accreditation

National Institutes of Health

IPA is a compliant member by the Office of Laboratory Animal Welfare (OLAW) of the the Department of Health and Human Services (HHS), USA and has been awarded with a 5-year Animal Welfare Assurance Number.

Canadian Council on Animal Care

IPA is a member of the the Canadian Council on Animal Care. The CCAC provides certification and policies on ethical animal care and use.

Better Business Bureau

IPA is a member of the Better Business Bureau. The BBB is a private, non-profit organization, dedicated to promoting fairness and honesty in the marketplace.

Citations

Peer Reviewed Publications

Review the expanding list of publications sourcing IPA's catalog products & exclusively developed custom mAb hybridomas.

Quick Links

Quote Request Form

To expedite receiving a quote from IPA for your custom antibody development project, fill in the on-line submission form or download the following form and email or fax to (250) 483-0309. Quotes issued in less than 24 hrs.

Product Order Form

To order any of IPA's antibody products, download the following form and email or fax to (250) 483-0309. Confirmation in less than 24 hrs.

Antigen Information Sheet

To initiate the immunization phase of your custom antibody development project, download the following form and submit it with the shipment of your antigen.

Custom options... In vivo or in vitro methods
Professional service... peace of mind
Quality assurance... GRP/GMP documented services

Service

In vitro - Bioreactor
Bioreactor cartridges provide the best alternative to using animals for custom monoclonal and hybridoma antibody production. One bioreactor cartridge can produce as much antibody as 10 to 12 ascites mice. In addition, the custom monoclonal and hybridoma antibodies in production in bioreactors contain fewer mouse proteins and nonspecific antibodies. Another advantage of our custom hybridoma and monoclonal antibody production is that some hybridoma cells do not grow and produce well in mice. In these cases the bioreactor system is an excellent animal-free alternative for custom bulk antibody production. 100 mL is the standard service.

Step 1: Materials needed from client for custom monoclonal and hybridoma antibody production: 1 x 106 - 1 x 107 hybridoma cells, viable, shipped on dry ice. IPA cultures these prior to inoculation of flask.	7 Days
Step 2: The membrane compartment is inoculated with 50 to 500 million cells, depending on the size of bioreactor, and incubated for about a week.	7 Days
Step 3: Supernatant rich with antibodies and cells (7.5 mL) are harvested twice per week (Mon and Thurs) after rapid growth is established. for a total of 15 mL per week.	7 Weeks
Step 4: The cells are removed by centrifugation and filtration and the antibody-containing supernatant is ready for use or further purification or modification.
Yield: 100 mL (25-50 mg, 100 in a perfect world), 1 mg/mL upper limit.	Total Time 9 weeks ($995 USD)

In vivo - Ascites fluid
Ascites fluid is often an ideal medium for the reliable production of custom monoclonal, hybridoma antibodies. Clients can take advantage of IPA?s full range of small and large scale custom ascites hybridoma and monoclonal antibody production services in diverse mouse and rat strains. Live or frozen cells may be sent to IPA for custom ascites, monoclonal and hybridoma antibody production. Traditionally, Antibody containing ascites fluid is then tapped from each mouse up to three times.

Step 1: Receipt and Expansion: Materials needed from client for custom monoclonal, hybridoma antibody production: 5 x 106 hybridoma cells, viable, shipped on dry ice. Upon receipt, all cell lines are assigned an Accession Number for tracking purposes. The cell line is transferred to a quarantine area where it is grown in culture and expanded to create cells ready for inoculation.	7 Days
Step 2: Inoculation Monoclonal antibodies are produced in bulk by implanting hybridoma cells into the peritoneal cavity of a primed mouse. Custom Ascites, Hybridoma and Monoclonal antibody production is initiated under cGMP guidelines.	7 Days
Step 3: Production Harvest Ascites is collected on wet ice and centrifuged to remove cellular and lipid material. The clarified ascites is placed into sterile containers, labeled, and immediately frozen.	5 Weeks
Yield: 5 mice provide 15 - 50 mL total (10 mg/mL average mouse ascites concentration).	7-8 weeks total production time ($415 USD)

Sample Submission

In Vitro (Integra BioReactor):

1 x 10⁶ - 1 x 10⁷ hybridoma cells, viable, shipped on dry ice. IPA cultures these prior to inoculation of flask.

In Vivo (ascites):

5 x 10⁶ hybridoma cells, viable, shipped on dry ice. The cell line is grown in culture and expanded to create cells ready for inoculation.

Confidentiality

IPA maintains all custom hybridoma and monoclonal antibody production information under the strictest confidentiality. All information and/or materials supplied by the client will be used as directed. Upon completion of the custom hybridoma or monoclonal antibody production project, all materials will be either returned or disposed of as directed by the client. IPA is willing to execute confidentiality agreements with its clients.

Copyright © 2011 ImmunoPrecise Antibodies Ltd.