New Services

High Affinity Screening

Never miss a high-affinity antibody again. Many methods are only applied after final antibody selection. ImmunoPrecise allows screening at a very early stage without need for pure, known quantities of mAb and without the need of Surface Plasmon Resonance and BEFORE any clones are disregarded during the early selection process.

Promotions

Amazon.com gift card Spring promotion

$100 Amazon.com gift card Spring promotion for every new custom mAb project started from Feb 1st until Jun 30th, 2011.

Accolades

Customer Feedback

Listen to what IPA's existing & past customers have to say about our products & custom antibody development services.

Accreditation

National Institutes of Health

IPA is a compliant member by the Office of Laboratory Animal Welfare (OLAW) of the the Department of Health and Human Services (HHS), USA and has been awarded with a 5-year Animal Welfare Assurance Number.

Canadian Council on Animal Care

IPA is a member of the the Canadian Council on Animal Care. The CCAC provides certification and policies on ethical animal care and use.

Better Business Bureau

IPA is a member of the Better Business Bureau. The BBB is a private, non-profit organization, dedicated to promoting fairness and honesty in the marketplace.

Citations

Peer Reviewed Publications

Review the expanding list of publications sourcing IPA's catalog products & exclusively developed custom mAb hybridomas.

Quick Links

Quote Request Form

To expedite receiving a quote from IPA for your custom antibody development project, fill in the on-line submission form or download the following form and email or fax to (250) 483-0309. Quotes issued in less than 24 hrs.

Product Order Form

To order any of IPA's antibody products, download the following form and email or fax to (250) 483-0309. Confirmation in less than 24 hrs.

Antigen Information Sheet

To initiate the immunization phase of your custom antibody development project, download the following form and submit it with the shipment of your antigen.

Rapid Prime / Standard Prime

Custom Monoclonal Antibody Development - Rapid Prime - Hybridoma

Rapid Prime - Rapid Prime is IPA’s trademark custom monoclonal antibody development service. This rapid immunization strategy saves weeks on custom hybridoma development. When coupled with our single-step cloning procedure, Clone-EZ, positive hybridoma cell lines are identified in 65 days. This animal friendly immunization strategy does not employ Freund's adjuvant and reduces the immunization phase from 84 days to only 18 days total. The Rapid Prime method has also been extremely useful for generating monoclonal antibodies against conformational epitopes.

For more information about Rapid Prime, click here.

For a comparison of features between Rapid Prime and Standard Prime, see the table below

Custom Monoclonal Antibody Development - Standard Prime

Standard Prime - IPA’s custom monoclonal antibody development service is fast and efficient. Utilizing IPA’s trademark single-step Clone-EZ Method, IPA shaves weeks off of the traditional time line for mAb production. IPA can custom develop monoclonal antibodies to an array of antigens including peptides, modified peptides, recombinant proteins, DNA plasmids and small molecules. If you need monoclonal antibodies even faster and have expressed or purified target molecules as antigen then consider our Rapid Prime Method click here.

For more information about Standard Prime, click here.

 

For a comparison of features between Rapid Prime and Standard Prime, see the table below

Production of Mouse Monoclonal Antibodies:
Rapid Prime vs Standard Immunization Method

	Advantages	Disadvantage
Standard method     4 female BALB/c mice are immunized Best responding 2 mice are used for fusion   Antigen amount required: 0.5mg minimum for immunizing (25ug/mouse approach) 1mg minimum for screening	1.     Gold standard – well characterized 2.     Theoretically produces higher affinity antibodies (longer immunization schedule allows for more affinity maturation and can produce high affinity antibodies) 3.     Test bleed done after 2nd boost to ensure appropriate  immune response is occurring 4.     Smaller amount of immunizing antigen required 5.     Back-up mice can be used for a repeat fusion if necessary 6.     Method available in rats	1.     Uses Freund's Adjuvant (category of invasiveness “D” according to CCAC*) 2.     Slower than RP         Approximately 115 days to     identify positive clones 3.     Intraperitoneal injections considered more invasive (mice can  have more health issues) 4.      Final pre-fusion boost is intravenous and can cause anaphylaxis 5.      Final pre-fusion boost cannot be IV for whole bacteria antigen     *Canadian Council on Animal Care
Rapid Prime (RP)     4 female BALB/c mice are immunized All 4 mice are used for fusion     Antigen amount required: 2mg minimum for immunizing 1mg minimum for screening	1.   Proprietary method developed in-house and used successfully for last 5 years 2.   Does not use Freund's adjuvant (considered more mouse friendly approach) 3.   Fast - approximately 65      days to identify positive clones 4.     Statistically creates more IgG antibodies 5.     Produces antibodies to conformational epitopes 6.     Works well for conserved and small proteins	1.     Could theoretically result in lower affinity antibodies 2.     Test bleed is not done prior to fusion. Localized immune response is created therefore immune sera may or may not be of value 3.     Immune sera can be low titer and therefore no positive control for ELISA assay (screening) 4.     Large amount of immunizing antigen required 5.     No back-up mice therefore no repeat fusion possible 6.     Used in mice only. Not available in rats

 

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