New Services

High Affinity Screening

Never miss a high-affinity antibody again. Many methods are only applied after final antibody selection. ImmunoPrecise allows screening at a very early stage without need for pure, known quantities of mAb and without the need of Surface Plasmon Resonance and BEFORE any clones are disregarded during the early selection process.

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Amazon.com gift card Spring promotion

$100 Amazon.com gift card Spring promotion for every new custom mAb project started from Feb 1st until Jun 30th, 2011.

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Accreditation

National Institutes of Health

IPA is a compliant member by the Office of Laboratory Animal Welfare (OLAW) of the the Department of Health and Human Services (HHS), USA and has been awarded with a 5-year Animal Welfare Assurance Number.

Canadian Council on Animal Care

IPA is a member of the the Canadian Council on Animal Care. The CCAC provides certification and policies on ethical animal care and use.

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IPA is a member of the Better Business Bureau. The BBB is a private, non-profit organization, dedicated to promoting fairness and honesty in the marketplace.

Citations

Peer Reviewed Publications

Review the expanding list of publications sourcing IPA's catalog products & exclusively developed custom mAb hybridomas.

Quick Links

Quote Request Form

To expedite receiving a quote from IPA for your custom antibody development project, fill in the on-line submission form or download the following form and email or fax to (250) 483-0309. Quotes issued in less than 24 hrs.

Product Order Form

To order any of IPA's antibody products, download the following form and email or fax to (250) 483-0309. Confirmation in less than 24 hrs.

Antigen Information Sheet

To initiate the immunization phase of your custom antibody development project, download the following form and submit it with the shipment of your antigen.

Quantitative proteomic immunoreagents for mass spectrometry applications

IPA advantage... Power of mAbs for quantitation
Specialty services... mAbs speficic for Mass Spectrometry
Professional experience... Highest success rate

ImmunoPrecise has proven expertise in developing custom reagents for use in Mass Spectrometry applications and has released two new antibody products for use in absolute quantitation. The large-scale systematic quantitation of proteins is an important component of proteomics and has contributed to the emergence of the new fields of systems biology and molecular-based medicine. Quantitative proteomics will provide new insights into biological function, facilitate the identification of diagnostic and surrogate disease markers, and contribute to the discovery of proteins as therapeutic targets.  Mass spectrometry (MS) coupled with shotgun style experiments has proven to be a powerful tool in the study of proteomes, but currently available technologies provide only relative quantitation information (rather than absolute). It will take more targeted approaches and specialized purpose-built reagents to truly expose the rich complexity of protein expression, modification, and interaction in biological systems. Immunoreagents for MS developed by ImmunoPrecise will facilitate larger, more sensitive, multiplexed studies that will advance protein diagnostics to the point of truly quantitative and absolutely specific assays.

 

Service. mAbs for mass spectrometry includes:

 

It starts with analysis of your target protein to identify candidate sequences and synthesize two different proteotypic peptides (based on trypsin digestion) compatible with quantitative mass spectrometry based research (free of residues subject to modification etc).

 

Once the two targets (to allow validation) are developed we use our Rapid Prime mAb development strategy and specialized high-throughput high-affinity screening methods to identify the best mAb candidates for MS use.

 

To assess the antibodies’ performance as quantitative pull down reagents and their compatibility and utility in mass spec analyses we test the mAb's ability to specifically recognize its target peptide and retain it during the process of mass spectrometric analysis

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Products. Current antibody products for quantitative mass spectrometry:

 

Thyroglobulin. A pair of monoclonal antibody reagents that each specifically capture a distinct trypsin proteotypic peptide. The antibodies were developed to capture the same tryptic peptides that would be generated in a typical sample preparation for MALDI-TOF or LC/MS/MS experiments.

 

Product name	target tryptic sequence	target species	antibody species	isotype
anti-Thyroglobulin-CPTAC-39c:	FSPDDSAGASALLR	human	mouse	IgG
anti-Thyroglobulin- CPTAC 39d	VIFDANAPVAVR	human	mouse	IgG

 

LPS Binding Protein. Same specifications as above, we offer a pair of monoclonal antibody reagents that each specifically capture a distinct trypsin proteotypic peptide for use in MALDI-TOF or LC/MS/MS experiments.

 

Product name	target tryptic sequence	target species	antibody species	isotype
anti-LPS-BP-Peptide PPI-1 b	ITLPDFTGDLR	human	mouse	IgG
anti-LPS-BP-Peptide PPI-1 c	LAEGFPLPLLK	human	mouse	IgG

 

Contact us for more information

 

Note* this service was made possible by a research grant fron the National Research Council of Canda (NRC/IRAP)

 

 

Confidentiality

IPA maintains all information under the strictest confidentiality. All information and/or materials supplied by the client will be used as directed. Upon completion of the project, all materials will be either returned or disposed of as directed by the client. IPA is willing to execute confidentiality agreements with its clients.

For more information about our methodology, click here.