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New Services

High Affinity Screening

Never miss a high-affinity antibody again. Many methods are only applied after final antibody selection. ImmunoPrecise allows screening at a very early stage without need for pure, known quantities of mAb and without the need of Surface Plasmon Resonance and BEFORE any clones are disregarded during the early selection process.

Promotions

Amazon.com gift card Spring promotion

$100 Amazon.com gift card Spring promotion for every new custom mAb project started from Feb 1st until Jun 30th, 2011.

Accolades

Customer Feedback

Listen to what IPA's existing & past customers have to say about our products & custom antibody development services.

Accreditation

National Institutes of Health

IPA is a compliant member by the Office of Laboratory Animal Welfare (OLAW) of the the Department of Health and Human Services (HHS), USA and has been awarded with a 5-year Animal Welfare Assurance Number.

Canadian Council on Animal Care

IPA is a member of the the Canadian Council on Animal Care. The CCAC provides certification and policies on ethical animal care and use.

Better Business Bureau

IPA is a member of the Better Business Bureau. The BBB is a private, non-profit organization, dedicated to promoting fairness and honesty in the marketplace.

ImmunoPrecise Antibodies Ltd. is a BBB Accredited Business. Click for the BBB Business Review of this Laboratories - Medical in Victoria BC
Citations

Peer Reviewed Publications

Review the expanding list of publications sourcing IPA's catalog products & exclusively developed custom mAb hybridomas.

Quick Links

Quote Request Form

To expedite receiving a quote from IPA for your custom antibody development project, fill in the on-line submission form or download the following form and email or fax to (250) 483-0309. Quotes issued in less than 24 hrs.

Product Order Form

To order any of IPA's antibody products, download the following form and email or fax to (250) 483-0309. Confirmation in less than 24 hrs.

Antigen Information Sheet

To initiate the immunization phase of your custom antibody development project, download the following form and submit it with the shipment of your antigen.


Custom Monoclonal Antibody Development

IPA advantage... Time-saving single-step cloning
Certified facility... Quality service guaranteed
Professional and experienced... High success rate


Custom Monoclonal Antibody Development - Rapid Prime


  • IPA Rapid Prime - Produce mAbs in 65 days *.
  • Certified facility - Quality service guaranteed.
  • Professional experience - Over 95% success rate.

Service


ImmunoPrecise’s proprietary Rapid Prime Method identifies positive mAbs in as little as 65 days. While this method also uses single-step cloning to accelerate the process, it is especially good for generating antibodies to conserved or smaller proteins, conformational epitopes and whole bacterial antigens.

 

We also offer a standard immunization procedure which is still accelerated by our single-step cloning method which also saves time compared to our competitors.

 

For Standard Prime Method Rapid Prime Method click here.

 

To compare Standard Method versus Rapid Prime Method refer to our advantage / disadvantage table on the monoclonal services page here

Rapid Prime - Custom Mouse Monoclonal Antibody Development

Project Phases

Time & Costing

I.               Immunization:

 

·          Immunization of 4 female BALB/c mice

·          Antigen requirements:

   -       For recombinant or purified molecules we require a total of 3mg of antigen

   -       Antigens in solution must be in PBS, neutral pH, at a concentration of 0.5 - 2mg/mL and contain nothing harmful to animals (no preservatives)

19    Days

II.             Fusion and Selection of Hybridomas

 

·          Lymphocytes from all 4 mice are pooled, purified then fused with SP2/0 myeloma

·          Fused cells are grown on HAT selective Clone-EZ semi-solid media for 10-12 days

·          Up to 948 hybridoma clones are transferred to 96-well plates and grown up for 4 days

16    days

III.            Screening of Hybridoma Clones

 

·          Primary screening of hybridoma tissue culture supernatants by ELISA using screening antigen

·          Positive clones are further tested by ELISA on screening antigen and negative control antigen (human transferrin) to confirm Ig secretion, specificity and to rule out false positives

·          Positive clones are isotyped and cultured for 32 days (post HAT selection) to identify stable expressing clones (Stability Period)

·          Tissue culture supernatants can be shipped to the Client for testing during the Stability Period  

30 days

IV.            Cryopreservation

 

·          Client can chose up to 5 positive clones with the option to purchase more

·          5 cryo-vials of each chosen clone plus 40mL of overgrown cell culture supernatant will be shipped to the Client by courier on dry ice upon completion

·          2 cryo-vials per clone will be stored in liquid nitrogen at IPA for a period of 1 month

·          Option to store cryo-vials long term at IPA is available

25 days

 Approx 90 days

Optional

 

V.             Antibody Concentration and Purification:

 

In vivo: Ascites production in BALB/c mice

-       The time period to produce ascites in mice is 6-8 week

 

In vitro: Bioreactor Flask

-       The time period to concentrate the antibody is 4-5 weeks

-        Protein G purification of ascites or bioreactor concentrate available

4 - 7 weeks

Prices available upon request

 

Confidentiality

IPA maintains all information under the strictest confidentiality. All information and/or materials supplied by the client will be used as directed. Upon completion of the project, all materials will be either returned or disposed of as directed by the client. IPA is willing to execute confidentiality agreements with its clients.


For more information about our methodology, click here.

Copyright © 2011 ImmunoPrecise Antibodies Ltd.