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Never miss a high-affinity antibody again. Many methods are only applied after final antibody selection. ImmunoPrecise allows
screening at a very early stage without need for pure, known quantities of mAb and without the need of Surface Plasmon Resonance
and BEFORE any clones are disregarded during the early selection process.
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$100 Amazon.com gift card Spring promotion for every new custom mAb project started from Feb 1st until Jun 30th, 2011.
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IPA is a compliant member by the Office of Laboratory Animal Welfare (OLAW) of the the Department of Health and Human Services (HHS), USA and has been awarded with a 5-year Animal Welfare Assurance Number. Canadian Council on Animal Care IPA is a member of the the Canadian Council on Animal Care. The CCAC provides certification and policies on ethical animal care and use. IPA is a member of the Better Business Bureau. The BBB is a private, non-profit organization, dedicated to promoting fairness and honesty in the marketplace. 
Review the expanding list of publications sourcing IPA's catalog products & exclusively developed custom mAb hybridomas.
To expedite receiving a quote from IPA for your custom antibody development project, fill in the on-line submission form or download the following form and email or fax to (250) 483-0309. Quotes issued in less than 24 hrs. To order any of IPA's antibody products, download the following form and email or fax to (250) 483-0309. Confirmation in less than 24 hrs. To initiate the immunization phase of your custom antibody development project, download the following form and submit it with the shipment of your antigen.   IPA advantage... Time-saving single-step cloning
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Standard Method - Custom Mouse or Rat Monoclonal Antibody Development |
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Project Phases |
Time & Costing |
I. Immunization:
· Immunization of 4 female BALB/c mice or 2 female F344 (SAS FISCH) rats - Test bleed is taken 7-10 days after 2nd boost - Antibody titer is evaluated by ELISA on screening antigen - Best responding 2 mice are chosen for fusion or best responding rat is chosen for fusion - Final boost (IV or IP) is given 4 days before fusing · Antigen requirements: - For recombinant or purified molecules we require a total of 3mg of antigen - Antigens in solution must be in PBS, neutral pH, at a concentration of 0.5 - 2mg/mL and contain nothing harmful to animals (no preservatives) · Synthetic Peptides 1mg of KLH conjugated peptide for immunization 2mg of BSA coupled peptide for screening |
73 Days |
II. Fusion and Selection of Hybridomas
· Lymphocytes from 2 mice are pooled, purified then fused with SP2/0 myeloma · Lymphocytes from 1 rat are purified and fused with either YB2/0 rat myeloma or X63Ag8 mouse myeloma · Fused cells are grown on HAT selective Clone-EZ semi-solid media for 10-12 days · Up to 948 hybridoma clones are transferred to 96-well plates and grown up for 4 days |
16 days |
III. Screening of Hybridoma Clones
· Primary screening of hybridoma tissue culture supernatants by ELISA using screening antigen · Positive clones are further tested by ELISA on screening antigen and negative control antigen (human transferrin) to confirm Ig secretion, specificity and to rule out false positives · Positive clones are isotyped and cultured for 32 days (post HAT selection) to identify stable expressing clones (Stability Period) · Tissue culture supernatants can be shipped to the Client for testing during the Stability Period |
30 days |
IV. Cryopreservation
· Client can chose up to 5 positive clones with the option to purchase more · 5 cryo-vials of each chosen clone plus 40mL of overgrown cell culture supernatant will be shipped to the Client by courier on dry ice upon completion · 2 cryo-vials per clone will be stored in liquid nitrogen at IPA for a period of 1 month · Option to store cryo-vials long term at IPA is available |
25 days |
Approximately 144 days |
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Optional
V. Antibody Concentration and Purification : In vivo: Ascites production in BALB/c mice for mouse hybdridoma - Ascites production in BALB/c Nude mice for rat hybridoma - The time period to produce ascites in mice is 6-8 weeks
In vitro: Bioreactor Flask - The time period to concentrate the antibody is 4-5 weeks - Protein G purification of ascites or bioreactor concentrate available |
4 - 7 weeks |
Confidentiality
IPA maintains all information under the strictest confidentiality. All information and/or materials supplied by the client will be used as directI. Immunization: · Immunization of 4 female BALB/c mice or 2 female F344 (SAS FISCH) rats - Test bleed is taken 7-10 days after 2nd boost - Antibody titer is evaluated by ELISA on screening antigen - Best responding 2 mice are chosen for fusion or best responding rat is chosen for fusion - Final boost (IV or IP) is given 4 days before fusing · Antigen requirements: - For recombinant or purified molecules we require a total of 3mg of antigen - Antigens in solution must be in PBS, neutral pH, at a concentration of 0.5 - 2mg/mL and contain nothing harmful to animals (no preservatives) · Synthetic Peptides 1mg of KLH conjugated peptide for immunization 2mg of BSA coupled peptide for screening 73 Days ed. Upon completion of the project, all materials will be either returned or disposed of as directed by the client. IPA is willing to execute confidentiality agreements with its clients.
For more information about our methodology, click here.
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