References

End-to-End Services

    Immunization

    2020


  • Anti-human TREM2 induces microglia proliferation and reduces pathology in an Alzheimer’s disease model, S. Wang et al., Journal of Experimental Medicine 2020.

  • 2019


  • Bacteriophage trigger antiviral immunity and prevent clearance of bacterial infection, J. Sweere et al., Science 2019.

  • Manufacturing

    2020

  • Mutational signature in colorectal cancer caused by genotoxic pks+ E. coli, C. Pleguezuelos-Manzano, et al., Nature, 2020.

  • CRISPR-Based Adenine Editors Correct Nonsense Mutations in a Cystic Fibrosis Organoid Biobank, M.H.Geurts, et al., Cell Stem Cell, 2020.

  • Outer membrane permeabilization by the membrane attack complex sensitizes Gram negative bacteria to antimicrobial proteins in serum and phagocytes, D.A.C. Heesterbeek, et al., BioRxiv, 2020.

  • Snake Venom Gland Organoids, Y. Post, H. Clevers, et al., Cell, 2020.

  • 2019


  • Novel Assays to Distinguish Between Properdin-Dependent and Properdin-Independent C3 Nephritic Factors Provide Insight Into Properdin-Inhibiting Therapy, M.A.H.M. Michels, et al., Frontiers in Immunology, 2019.

  • Enhanced Antiviral Activity of Human Surfactant Protein D by Site-Specific Engineering of the Carbohydrate Recognition Domain, M. van Eijk, et al., Frontiers in Immunology, 2019.

  • Insights Into Enhanced Complement Activation by Structures of Properdin and Its Complex With the C-Terminal Domain of C3b, R.M. van den Bos, et al., Frontiers in Immunology, 2019.

  • Tubuloids derived from human adult kidney and urine for personalized disease modeling, F. Schutgens, et al., Nature Biotechnology, 2019.

  • Affibody-Mediated Sequestration of Amyloid β Demonstrates Preventive Efficacy in a Transgenic Alzheimer’s Disease Mouse Model, A. Boutajangout, et al., Frontiers Aging Neuroscience, 2019.

  • A novel L1CAM isoform with angiogenic activity generated by NOVA2-mediated alternative splicing, F. Angiolini et al., eLife, 2019.

  • Complement-dependent outer membrane perturbation sensitizes Gram-negative bacteria to Gram-positive specific antibiotics, D.A.C. Heesterbeek, et al., Scientific Reports, 2019.

  • Four individually druggable MET hotspots mediate HGF-driven tumor progression, C. Basilico, et al., The Journal of Clinical Investigation 2019.

  • Analytical characterization and reference interval of an enzyme-linked immunosorbent assay for active von Willebrand factor, L.N. van der Vorm, et al., PLOS ONE, 2019.

  • Bacterial killing by complement requires membrane attack complex formation via surface-bound C5 convertases, D.A.C. Heesterbeek, et al., The EMBO Journal, 2019.

  • Anti-LRP5/6 VHHs promote differentiation of Wnt-hypersensitive intestinal stem cells, N. Fenderico, et al., Nature Communications,.

  • Structure and flexibility of the extracellular region of the PirB receptor, H.C. Vlieg, et al., JBC, 2019.

  • 2018


  • Mix and match of the tumor metastasis suppressor Nm23protein isoformsin vitroandin vivo, C.M. Potel, et al., FEBS, 2018.

  • IL4-10 fusion protein has chondroprotective, anti-inflammatory and potentially analgesic effects in the treatment of osteoarthritis, C. Steen-Louws, et al., Osteoarthritis and Cartilage, 2018.

  • Unbiased Combinatorial Screening Identifies a Bispecific IgG1 that Potently Inhibits HER3 Signaling via HER2-Guided Ligand Blockade, C.A.W.Geuijen, et al., Cancer Cell, 2018..

  • Structural basis of latent TGF-β1 presentation and activation by GARP on human regulatory T cells, S. Liénart, et al., Science, 2018.

  • CXCR4-targeting nanobodies differentially inhibit CXCR4 function and HIV entry, A. Van Hout, et al., Biochemical Pharmacology, 2018.

  • Nanobody-Fc constructs targeting chemokine receptor CXCR4 potently inhibit signaling and CXCR4-mediated HIV-entry and induce antibody effector functions, V. Bobkov, et al., Biochemical Pharmacology, 2018.

  • Cryo-EM structures of human STEAP4 reveal mechanism of iron(III) reduction, W. Oosterheert, et al., Nature communications, 2018.

  • Structures of Teneurin adhesion receptors reveal an ancient fold for cell-cell interaction, V.A. Jackson, et al. Nature communications, 2018.

  • A bispecific antibody strategy to target multiple type 2 cytokines in asthma, M. Godar, et al., The journal of allergy and clinical Immkunology, 2018.

  • An Approach to Incorporate Multi-Enzyme Digestion intoC-TAILS for C-Terminomics Studies, Y. Zhang, et al., Proteomics, 2018.

  • Functional Characterization of Alternative and Classical Pathway C3/C5 Convertase Activity and Inhibition Using Purified Models, S.A. Zwarthoff, et al., Frontiers in Immunology, 2018.

  • Structural insights into SorCS2–Nerve Growth Factor complex formation, N. Leloup, et al., Nature Communications, 2018.

  • Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3, L. Scietti, et al., Nature Communications, 2018.

  • Structures of C1-IgG1 provide insights into how danger pattern recognition activates complement, D. Ugurlar, et al., Science, 2018.

  • A rationally designed NRP1-independent superagonist SEMA3A mutant is an effective anticancer agent, N. Gioelli, et al., Science Translational Medicine 2018.

  • Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus, M. van Eijk, et al., Journal of Biological Chemistry, 2018.

  • 2017

  • Nogo Receptor crystal structures with a native disulfide pattern suggest a novel mode of self‐interaction, M.F. Pronker, et al., cts Crystallographica Section D, 2017.

  • A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels, S.A. Khetarpal, et al., Nature Medicine, 2017..

  • Stochastic palmitoylation of accessible cysteines in membrane proteins revealed by native mass spectrometry, R.N.P. Rodenburg, et al., Nature Communications, 2017.

  • Recombinant Expression of the Full-length Ectodomain of LDL Receptor-related Protein 1 (LRP1) Unravels pH-dependent Conformational Changes and the Stoichiometry of Binding with Receptor-associated Protein (RAP), C. De Nardis, et al., Journal of Biological Chemistry, 2017.

  • Regulator-dependent mechanisms of C3b processing by factor I allow differentiation of immune responses, X. Xue , et al., Nature Structural and Molecular Biology, 2017.

  • Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release, N. Leloup, et al., Nature Communications, 2017.

  • Surrogate Wnt agonists that phenocopy canonical Wnt and β-catenin signalling, C.Y. Janda, et al., Nature, 2017..

  • 2016


  • Dual Constant Domain-Fab: A novel strategy to improve half-life and potency of a Met therapeutic antibody, S. Cignettoa, et al., Molecular Oncology, 2016.

  • IL4-10 Fusion Protein Is a Novel Drug to Treat Persistent Inflammatory Pain, N. Eijkelkamp, et al., The Journal of Neuroscience, 2016.

  • Molecular Basis of Assembly and Activation of Complement Component C1 in Complex with Immunoglobulin G1 and Antigen, G. Wang, et al., Molecular Cell, 2016.

  • Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis, B. Banushi, et al., Nature Communications, 2016.

  • A live RSV vaccine with engineered thermostability is immunogenic in cotton rats despite high attenuation, C.C. Stobart, et al., Nature Communications, 2016.

  • Structural Mimicry of Receptor Interaction by Antagonistic Interleukin-6 (IL-6) Antibodies, C. Blanchetot, et al., JBC, 2016.

  • DNA immunization combined with scFv phage display identifies antagonistic GCGR specific antibodies and reveals new epitopes on the small extracellular loops, B. van der Woning, et al., mAbs vol. 8, no. 6, 1126–1135, 2016.

  • Structural basis of myelin-associated glycoprotein adhesion and signalling, M.F. Pronker, et al., Nature Communications, 2016.

  • 2015


  • Depleting MET-Expressing Tumor Cells by ADCCProvides a Therapeutic Advantage over Inhibiting HGF/MET Signaling, A. Hultberg, et al., Cancer Research, 2015.

  • Cryoelectron Tomography of the NAIP5/NLRC4 Inflammasome: Implications for NLR Activation, C.A. Diebolder, et al., Structure, 2015.

  • Structural basis for inhibition of TLR2 by staphylococcal superantigen-like protein 3 (SSL3), K.J. Koymans, et al., PNAS, 2015..

  • Molecular insights into the surface-specific arrangement of complement C5 convertase enzymes, E.T. Berends, et al., BMC Biology, 2015.

  • Olfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure, M.F. Pronker, et al., J. Biol. Chem. 2015 Jun 12; 290(24): 15092–15101

  • 2014


  • Multimeric Growth Hormone Receptor Complexes Serve as Signaling Platforms, M. Sedek, et al., JBC, 2014

  • Plasmin Cleavage of von Willebrand Factor as an Emergency Bypass for ADAMTS13 Deficiency in Thrombotic Microangiopathy, C. Tersteeg, et al., Circulation, 2014

  • Four individually druggable MET hotspots mediate HGF-driven tumor progression, C. Basilico, et al., J Clin Invest, 2014.

  • Novel Sequence Variation Affects GPIbα in Post-diarrheal Hemolytic Uremic Syndrome, E. Volokhina, et al.,

  • Ensemble refinement shows conformational flexibility in crystal structures of human complement factor D, F. Forneris, et al., Acta Crystallogr. D Biol. Crystallogr., 2014.

  • 2013


  • Molecular imaging with a fluorescent antibody targeting carbonic anhydrase IX can successfully detect hypoxic ductal carcinoma in situ of the breast, A.S.A. van Brussel, et al., Breast Cancer Research and Treatment, 2013.

  • TLR Accessory Molecule RP105 (CD180) Is Involved in Post-Interventional Vascular Remodeling and Soluble RP105 Modulates Neointima Formation, J.C. Karper, et al., PLOS one, 2013

  • Near-Infrared Fluorescence Molecular Imaging of Ductal Carcinoma In Situ with CD44v6-Specific Antibodies in Mice: A Preclinical Study, J.F. Vermeulen, A.S.A. van Brussel, et al., Mol Imaging Biol., 2013, 15(3), 290 - 298.

  • Towards Establishing a Rhinoceros-Specific Interferon-Gamma (IFN-γ) Assay for Diagnosis of Tuberculosis, D. Morar, et al., Transboundary and Emerging Diseases, (60), Suppl 1: 60 - 60.

  • Structures of Wnt-Antagonist ZNRF3 and Its Complex with R-Spondin 1 and Implications for Signaling, W.C. Peng, et al., Plos one, 8(12), e83110.

  • The elephant interferon gamma assay: a contribution to diagnosis of tuberculosis in elephants, T. Angkawanish, et al., Transboundary and Emerging Diseases, (60), Suppl 1: 53 - 59.

  • Structure of Stem Cell Growth Factor R-spondin 1 in Complex with the Ectodomain of Its Receptor LGR5, W.C. Peng, et al., Cell Reports, 3 (6), 1885 - 1892.

  • 2012


  • Antibody engineering reveals the important role of J segments in the production efficiency of llama single-domain antibodies in Saccharomyces cerevisiae, A. Gorlani, et al., PEDS, 2012.

  • Novel C3 mutation p.Lys65Gln in aHUS affects complement factor H binding, E. Volokhina, et al., Pediatric Nephrology, 27, 1519 - 1524.

  • Fusion of the Fc part of human IgG1 to CD14 enhances its binding to Gram-negative bacteria and mediates phagocytosis by Fc receptors of neutrophils, A. Vida, et al., Immunology letters, 146, 31 - 39.

  • Near-Infrared Fluorescence Molecular Imaging of Ductal Carcinoma In Situ with CD44v6-Specific Antibodies in Mice: A Preclinical Study, J.F. Vermeulen, et al., Molecular Imaging and Biology, 2012.

  • Formation and Structure of a NAIP5-NLRC4 Inflammasome Induced by Direct Interactions with Conserved N- and C-terminal Regions of Flagellin, E.F. Halff, et al., JBC, 287, 38460 - 38472.

  • Development of a lion-specific interferon-gamma assay, M. Maas, et al., Veterinary Immunology and Immunopathology, 149, (3–4), 292–297.

  • 2011


  • Humanization and Characterization of an Anti-Human TNF-α Murine Monoclonal Antibody, Chiu, et al., PLoS One. 2011; 6(1): e16373.

  • Enhanced secretion of leukocyte‐associated immunoglobulin‐like receptor 2 (LAIR‐2) and soluble LAIR‐1 in rheumatoid arthritis: LAIR‐2 is a more efficient antagonist of the LAIR‐1–collagen inhibitory interaction than is soluble LAIR‐1, M.J.M. Olde Nordkamp, et al., Rheumatoid Arthritis, 2011.

  • Introduction of N-linked glycans in the lectin domain of surfactant protein D (SP-D): impact on interactions with influenza A viruses, M. van Eijk, et al., JBC, 286, 20137 - 20151.

  • Exploring New Proteome Space: Combining Lys-N Proteolytic Digestion and Strong Cation Exchange (SCX) Separation in Peptide-Centric MS-Driven Proteomics, N. Taouatas, et al., Methods in Molecular Biology, 753, 157-167.

  • 2010


  • Insights into complement convertase formation based on the structure of the factor B-cobra venom factor complex, B.J.C. Janssen, et al., EMBO, 28(16), 2469–2478

  • Structures of C3b in Complex with Factors B and D Give Insight into Complement Convertase Formation, F. Forneris, et al., Science, 330 (6012), 1816–1820.

  • A Novel Disulfide Pattern in Laminin-Type Epidermal Growth Factor-like (LE) Modules of Laminin β1 and γ1 Chains, S. Kalkhof, et al., Biochemistry, 49, 8359–8366.

  • Clathrin is essential for meiotic spindle function in oocytes, J.J. Holzenspies, et al., Reproduction, 140, 223-233.

  • The influenza A virus hemagglutinin glycosylation state affects receptor-binding specificity, R.P. de Vries, et al., Virology, 403, 17-25.

  • Studies in zebrafish reveal unusual cellular expression patterns of gonadotropin receptor messenger ribonucleic acids in the testis and unexpected functional differentiation of the gonadotropins, A. Garcia-Lopez, et al., Endocrinology, 151 (5), 2349-2360.

  • Purification of parvalbumin from Carp: A protocol that avoids heat-treatment, S.J. Koppelman, et al., Journal of food Science, 75 (3), T49-T56.

  • Molecular Cloning and Functional Characterization of a Zebrafish Nuclear Progesterone Receptor, S.X. Chen, et al., Biology of Reproduction, 82 (1), 171-181.

  • 2009


  • Structural and functional implications of the alternative complement pathway C3 convertase stabilized by a staphylococcal inhibitor, S.H.M. Rooijakkers, et al., Nature Immunology, 10 (7), 721-729.

  • Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis, P.P. de Waal, et al., Journal of Endocrinology, 202, 287-297.

  • Staphylococcal SSL5 inhibits leukocyte activation by chemokines and anaphylatoxins, J. Bestebroer, et al., Blood, 113 (2), 328-337.

  • Insights into complement convertase formation based on the structure of the factor B-cobra venom factor complex, B.J.C. Janssen, et al.,EMBO, 28, 2469-2478.

  • Staphylococcal Superantigen-like10 Inhibits CXCL12-Induced Human Tumor Cell Migration, A.M.E. Walenkamp, et al., 11 (4), 333-344.

  • Structure and functional relevance of the Slit2 homodimerization domain, E. Seiradake, et al., EMBO, 10, 736-741.

  • 2008


  • cGMP-binding Prepares PKG for Substrate Binding by Disclosing the C-terminal Domain, V. Alverdi, e tal., Journal of Molecular Biology, 104 (38), 14923-14928.

  • Chicken lung lectin is a functional C-type lectin and inhibits haemagglutination by influenza A virus, A. Hogenkamp, et al., Veterinary Microbiology, 130, 37-46.

  • Platelet activation by dimeric beta2-glycoprotein I requires signaling via both glycoprotein Ibalpha and apolipoprotein E receptor 2', R.T. Urbanus, et al., Journal of Thrombosis and Haemostasis, 6 (8), 1405 - 1412.

  • In vitro and in vivo bioactivity of recombinant canine hepatocyte growth factor, B. Arends, et al., The Veternary Journal, 178, 70-77.

  • Crystal structures of TAFI elucidate the inactivation mechanism of activated TAFI; A novel mechanism for enzyme auto-regulation, P.F. Marx, et al., Blood, 112 (7), 2803-2809.

  • Cathepsin L functionally cleaves the SARS-CoV class I fusion 1 protein upstream of rather than adjacent to the fusion peptide, B.J. Bosch, et al., Journal of Virology, 82 (17), 8887-8890.

  • Cathepsin L functionally cleaves the SARS-CoV class I fusion 1 protein upstream of rather than adjacent to the fusion peptide, B.J. Bosch, et al., Journal of Virology, 82 (17), 8887-8890.

  • 2007


  • Factor B structure provides insights into activation of the central protease of the complement system, F.J. Milder, et al., Nature Structural & Molecular Biology, 14, 224 - 228.

  • Surface-Plasmon-Resonance-Based Chemical Pteomics: Efficient Specific Extraction and Semiquantitative Identification of Cyclic Nucleotide-Binding Proteins from Cellular Lysates by Using a Combination of Surface Plasmon Resonance, Sequential Elution and Liquid Chromatography-Tandem Mass Spectrometry, N.F.C. Visser, et al., ChemBioChem, 8, 298 - 305.

  • The Beta-Defensin Gallinacin-6 Is Expressed in the Chicken Digestive Tract and Has Antimicrobial Activity against Food-Borne Pathogens, A. van Dijk, et al, Antimicrobal agents and chemotherapy, 51-3, 912-922.

  • Platelets express three different splice variants of ApoER2 that are all involved in signaling, M.T.T. Pennings, et al., Journal of Thrombosis and Haemostasis, 5, 1538 - 1544.

  • Cloning, expression, crystallization and preliminary X-ray analysis of the first two Ig domains from human Roundabout 1 (Robo1), C. Morlot, et al., Acta Cryst. F63, 689 - 691.

  • Production of Slit2 LRR domains in mammalian cells for structural studies and the structure of human Slit2 domain 3, C. Morlot, et al., Acta Crystallogr D. Biol Crystallography, Sep., 63(Pt 9), 961-968.

  • Structural insights into the Slit-Robo complex, C. Morlot, et al.,PNAS, 104 (38), 14923-14928.

  • 2006


  • Interaction of ß2-glycoprotein I with members of the low density lipoprotein receptor family, M.T.T. Pennings, et al., Journal of Thrombosis and Haemostasis, 4, 1680 - 1690.

  • Origin and evolution of the peroxisomal proteome, Toni Gabaldón, et al.,Biology Direct 2006, 1:8 (23 March 2006).

  • Analysis of the cGMP/cAMP Interactome Using a Chemical Proteomics Approach in Mammalian Heart Tissue Validates phingosine Kinase Type 1-interacting Protein as a Genuine and Highly Abundant AKAP, Arjen Scholten, et al., Journal of Proteome Research 2006, 5, 1435-1447.

  • Characterization of bitiscetin-2, a second form of bitiscetin from the venom of Bitis arietans: comparison of its binding site with the collagen-binding site on the von Willebrand factor A3-domain, B. Obert, et al., Journal of Thrombosis and Haemostasis, 4, 1596-1601.

  • VWF A1 domain can adequately substitute for A3 domain in recruitment of flowing platelets to collagen, Arnaud Bonnefoy, et al., Journal of Thrombosis and Haemostasis, 4, 2151 - 2161.

  • Structure of Complement Component C2a: Implications for Convertase Formation and Substrate Binding, Fin J. Milder, et al., Structure, 14, 1587-1597.

  • 2005


  • The binding site in beta2-glycoprotein I for apoER2' on platelets is located in domain V, M. van Lummel, et al., JBC, 280(44), 36729-3673.

  • 2003

  • Mapping the collagen-binding site in the von Willebrand Factor A3-domain. R.A. Romijn, et al., Journal of Biological Chemistry, 278(17), 15035-15039.

  • 2002

  • Structures of Glycoprotein Iba and its complex with von Willebrand Factor A1 domain. et al., Science, 2002, 297, 1176-1179.

  • 2001

  • Identification of the Collagen-binding Site of the von Willebrand Factor A3-domain. R.A. Romijn, et al., Journal of Biological Chemistry, 2001, 276(13), 9985-9991.