ImmunoPrecise’s proprietary Rapid Prime™ method identifies positive monoclonal antibodies in as little as 32 days. This method is especially useful for generating anti-idiotypic antibodies and has been extremely useful for producing monoclonal antibodies against conformational epitopes. Other sources of immunogens include conserved or smaller proteins, peptides, and whole cell bacteria.
After the immunization of rodents, we apply our proprietary electrofusion or PEG-fusion to generate hybridomas. The subsequent cloning, using semi-solid media and clone picking to accelerate the process, allows us to identify monoclonal antibody producing hybridoma cells in a very short time.
Rapid Prime immunization can also be combined with B cell Select or Phage Display.