ImmunoProtect™: Hybridoma Sequencing
Your monoclonal antibody (mAb) is extremely valuable, but hybridoma cell lines can become unstable over time and can stop secreting antibody. With ImmunoProtect™, the DNA sequence of your antibody will be determined and can be used to express your mAb recombinantly, should your hybridoma cell line become unstable.
ImmunoPrecise offers the following antibody optimization services:
Our antibody sequencing, cloning, reformatting, and expression service can clone and express any variable antibody domain into any immunoglobulin context required, such as full size mouse, rabbit, chicken, and human format; or chimeras with any Fc region of choice, including effector mutants or Fc fusions.
Animal-derived monoclonal antibodies can elicit human anti-animal antibody responses in patients. Therefore it is usually necessary to replace the animal content with the corresponding human amino acid residues. We provide a robust and efficient antibody humanization service, which consistently retains affinity and specificity levels. Our royalty free approach is based on state of the art in silico antibody modeling to identify essential framework and CDR residues for grafting onto a human antibody framework.
Antibody affinity maturation
Antibody affinity is important in therapeutic and diagnostic applications. ImmunoPrecise’s antibody affinity maturation service can improve antibodies affinities by several fold. We can apply different strategies to increase the affinity of your antibody, including gene shuffling and random mutagenesis.
|I. DNA Sequencing
Phase I Deliverables
|II. Recombinant Antibody Binding Confirmation and Test Expression
Phase II Deliverables
As with all our services, we will tailor an antibody sequencing package for your specific needs. Contact us to discuss your hybridoma health, stability or antibody secretion challenges.